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Essentials of Immunology and Serology
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Chapter Electrophoresis. We were unable to find this edition in any bookshop we are able to search. More filters.
Radial immunodiffusion RID or Mancini method, Mancini immunodiffusion or single radial immunodiffusion assay, is an immunodiffusion technique used in immunology to determine the quantity or concentration of an antigen in a sample. A solution containing antibody is added to a heated medium such as agar or agarose dissolved in buffered normal saline. The molten medium is then poured onto a microscope slide or into an open container, such as a Petri dish , and allowed to cool and form a gel. The antigen diffuses radially into the medium, forming a circle of precipitin that marks the boundary between the antibody and the antigen. Antigen-antibody complexes are small and soluble when in antigen excess.
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